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Development of a multiplex digital DiRT-PCR to test for potato viruses and blackleg bacteria directly on the same tuber sap (Digi-Test)
Project
Project code: A/18/11
Contract period: 01.06.2016
- 30.04.2021
Budget: 314,660 Euro
Purpose of research: Applied research
Keywords: potato virus, blackleg, qPCR, multiplex
Development of a suitable diagnostic test that fulfills following aims:
- nucleic acid based detection of viruses and bacteria (real-time polymerase chain reaction)
- direct test on potato tuber sap (DiRT-PCR)
- detection of multiple viruses and bacteria at the same time (multiplex DiRT-PCR)
- possibility to test several single tubers in one reactin (pool testing).
Section overview
Subjects
- Crop Production
- Crop Protection