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Priming for enhanced defense as a strategy to optimize crop resistance and as a possible breeding target (PrimedPlant)
Project
Project code: JKI-RS-08-3398
Contract period: 01.01.2017
- 30.09.2019
Purpose of research: Applied research
The main objective of PrimedPlant is to harness the priming capacities of barley plants using the soil microbiome(s). To achieve this we will use the genetic diversity of barley on the one hand, and the functional diversity of the rhizosphere microbiome on the other. Latest research showed that some microorganisms induced priming in plants. Since primed plants are able to fight a pathogen’s attack better than naïve plants, our goal here is to use this natural potential of barley plants and combine it with priming-inducing competency of plant-associated microorganisms. Our PrimedPlant project is based on two main features: i) The fact that members of the soil microbiome influence plant defence mechanisms by either induction of systemic resistance (ISR) or primed state (priming), and; ii) Unbiased screen of genetically diverse barley lines. The combination of priming-responsive genome(s) with resistance- or priming-inducing microbiome(s) has great potential. It is the key innovation of our approach, since such directed screening for priming inducing capacities in barley has not been performed. Results gathered in PrimedPlant should facilitate several new approaches ranging from new application of biologicals in agriculture to barley lines available for breeding companies. In addition, we expect to obtain new findings in the field of plant-bacteria interactions, especially regarding the role of plant immune system in the outcome of the interaction between beneficial bacteria, harmful microorganisms and the host plant. At the same time the knowledge about the principal components of the priming-inducing community (or communities) would allow directed soil enrichment with those biologicals. The mid- and longterm goals of the project would be a new breeding strategy, including the priming-efficient barley lines as well as the transfer of this knowledge to wheat and maize.
In order to reach our main goal we divided the initial three years of the project into work packages with distinct tasks and milestones. 1. Characterization of the primed state in barley at physiological and molecular levels. Based on our knowledge obtained from Arabidopsis studies and data collected in barley and wheat, we will analyze the signaling pathways relevant for establishment of priming and develop molecular markers for its detection. 2. Identification of barley lines highly responsive to priming by microbiomes and the genomic regions/genes involved. The demanding task of the screen for priming induction will be performed at IPK Gatersleben and JKI Quedlinburg. We will use the automated DPPN platform. 3. Taxonomical characterization of the functional components in priming-inducing microbial communities. We will analyze bacteria associated with the rhizosphere of plants grown in about 20 different soil types. 4. Assessing the priming activity of beneficial endophytic fungi and their associated bacteria from the Sebacinalean symbiosis. To increase our chances of success we will analyze 25 newly isolated strains from the genera Piriformospora (Serendipita) recently collected from Sorghum fields in Congo. During the first phase (initial three years) of the project we aim to achieve three main milestones; a) Collection of priming-responsive barley lines, which could be taken over to the next stage of the project (genetics association and breeding strategy for new barley varieties); b) The characterization of the microbial community responsible for the priming effect, strains which could be used in the new product(s) and; c) A better understanding of the physiological state defined as 'primed plant'.
https://doi.org/10.1094/PBIOMES-09-18-0041-R
https://doi.org/10.1094/PBIOMES-03-19-0015-R
Section overview
Subjects
- Plant Breeding
- Crop Protection
Framework programme
Funding programme
Excutive institution
Institute of Resistance Research and Stress Tolerance (JKI-RS)
Participating institutions
Funding institution
Project management agencies
Associated projects: PrimedPlant
- Collaborative project: Priming for enhanced defense as a strategy to optimize crop resistance and as a possible breeding target. Subproject D
- Collaborative project: Priming for enhanced defense as a strategy to optimize crop resistance and as a possible breeding target. Subproject A
- Priming for enhanced defence as a stategy to optimize crop resistance and as a possible breeding target