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Proteomic analysis of cellular changes after treatment with contact allergens in human monocytic cell lines and in primary dendritic cells. 2017: Transcriptomic and proteomic Analysis of T cells and monocyte-derived dendritic cells
Project
Project code: BfR-CPS-08-1322-470
Contract period: 01.03.2011
- 31.12.2017
Purpose of research: Applied research
A wide range of chemicals may induce allergic contact dermatitis. In the development of allergic contact dermatitis two phases can be separated: sensitization and elicitation. During sensitization contact allergens or sensitizers penetrate through the skin barrier and interact with epidermal dendritic cells, which then become activated and migrate into local lymph nodes to induce T cell-mediated immune responses. Sensitizers belong to different classes of chemicals that hardly share any structural homologies. Chemical sensitizers often cause oxidative stress thereby inducing the cytoprotective Keap1/Nrf2 pathway. Here we use different proteomics approaches to monitor changes in different cellular compartments as well as on the level of membrane proteins. It is well known that upon activation of dendritic cells the protein composition of the cell membrane becomes altered. The proteomics approaches proposed will allow for identification of new, as yet unknown proteins in the context of contact allergy. The goal will be to better understand cellular changes triggered by chemicals in dendritic cells during sensitization and to identify novel biomarkers. In the development of in vitro test systems for sensitization the most likely outcome will be the requirement of a combination of a range of different assays, each of which mirroring one particular molecular feature contributing to contact allergy development.
Section overview
Subjects
- Biotechnology