We use cookies on our website. Some are necessary for the operation of the website. You can also allow cookies for statistical purposes. You can adjust the data protection settings or agree to all cookies directly.
Data Protection Declaration
Imprint
Delivery of Ribonucleoproteins through Cavitation Bubble Induced Shock Wave and Improved Event Screening for Fast Regeneration of Abiotic-Stress Tolerant Crop Plants (ASTRA)
Project
Project code: 031B0536
Contract period: 01.07.2018
- 30.06.2020
Budget: 405,044 Euro
Purpose of research: Experimental development
The goal of the project is to develop and establish a new technique for the ribonucleoprotein (RNP) introduction and selection of the events to create non-GM abiotic stress like cold, drought, and salinity-tolerant crop plants within a short period of time. We want to develop a new method called the "cavitation bubble-induced shock wave-mediated RNP introduction" into plant cells that might cause less damage to the cells, and therefore result in high regeneration efficiency of the mutated plants. When using RNP, no marker genes are introduced into the genome and therefore screening for mutations is laborious and time consuming. In this project, we will attempt to solve this problem using a fluorescent dye attached to RNP, and then select cells that have fluorescent RNP using a micromanipulator. Further screen the mutated plants using high resolution Melting-Curve analysis for simple and fast detection of the mutation, and confirmed by Sanger sequencing. The selected plants will be grown in the greenhouse for the development of T2 homozygous mutants. The developed T2 homozygous plants will be further subjected to abiotic stress tolerance analysis to identify the best genotypes.
Section overview
Subjects
- Plant Breeding
- Process engineering
