We use cookies on our website. Some are necessary for the operation of the website. You can also allow cookies for statistical purposes. You can adjust the data protection settings or agree to all cookies directly.
Data Protection Declaration
Imprint
Biodiversity of mycotoxin producing fungi from fruits and vegetables
Project
Project code: MRI-OG-08-052
Contract period: 01.01.2001
- 01.12.2017
Purpose of research: Applied research
Mycotoxin producing fungi or fruit spoiling fungi, like Alternaria, Penicillium or Aspergillus often constitute inhomogeneous species with variable characteristics. With the aid of molecular typing methods, like RAPD, AFLP or rDNA sequencing it is possible to analyse differences at the genotypic level and to form population groups according to these differences. Features like mycotoxin biosynthesis and colonization capacity may correlated with these genotypic groups. Knowledge about the distribution of these groups on foods and vegetables enable the prediction of the risk of contamination and mycotoxin biosynthesis in relation to specific products and help to develop novel measures against mycotoxin biosynthesis.
P. digitatum and P. italicum strains from citrus fruits were isolated and characterized by RAPD. Species specific RAPD pattersn could be identified. From single bands SCAR primers could be developed. Furthermore a RAPD heterogeneity could be identified on the species level. Also after restriction digestion of ITS regions genetic differences between the species could be found. Further analysis of ochratoxin A producing fungi showed a clear correlation of the RAPD genotype and the ability to produce ochratoxin A, which demonstrates the usefulness of the approach.
Section overview
Subjects
- Biotechnology
- Food microbiology
Framework programme
Funding programme
Excutive institution
MRI - Department of Safety and Quality of Fruit and Vegetables (MRI-OG)
