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Collaborative project: Molecular charcterization of different TRV isolates and analysis of the interaction between virus, nematode and potato to improve resistance breeding. Subproject 4 (STEP-4-STEP)
Project
Project code: 2818208215, JKI-EP-08-2280
Contract period: 11.04.2017
- 10.04.2020
Budget: 293,762 Euro
Purpose of research: Applied research
Spraing symptoms on potato tubers appear particularly in moist and warmer years and when grown on light and/or organic soils. Besides spraing symptoms on the surface tubers often show ring necrosis in the tuber. As a result, even slightly affected potatoes can no longer be marketed as edible or refined potatoes. The cause of these symptoms is Tobacco rattle virus (TRV), which is transmitted by ectoparasitic nematodes of the genera Trichodorus and Paratrichodorus. The genome of the TRV consists of RNA1 and RNA2 strands. The composition of the RNA bases can vary greatly depending on the geographic origin of the isolates. Besides, potato varieties can differ significantly in their resistance to TRV, from highly susceptible to completely resistant. However, the resistance expression depends not only on the potato cultivar, but also on the molecular properties of the respective TRV isolate and its interaction with the virus vector. In the proposed research project, the molecular properties of TRV strains occurring in different regions of Germany are to be analyzed. Based on this, investigations are carried out on the spread, symptomatology and infectivity of the different TRV isolates. The trichodorids occurring in the different regions are determined and tested for being loaded with TRV. The interaction between TRV isolate, virus vector and potato is carried out in cooperation with the breeders involved using selected potato cultivars at different field sites. The results of this work will allow a more detailed resistance assessment of potato genotypes so that a more targeted selection of crossing partners becomes possible. Corresponding crosses could provide the basis for a marker-assisted selection on TRV resistance in the long term. Corky ringspot on potato is caused by the Tobacco rattle virus (TRV). TRV is transmitted by plant-parasitic nematodes of the genera Trichodorus and Paratrichodorus. There are no pesticides available to control the disease. Therefore, the aim is to breed resistant potato cultivars. During the reporting period we accomplished the following: 1) Confirmation of visual disease screening by PCR, 2) verification of dependency of the TRV genome variability on location, 3) detection and identification of (vector) nematodes species and TRV isolates found at the various locations, 4) further activities to establish pure nematode cultures.
Section overview
Subjects
- Plant Breeding
- Crop Protection
Framework programme
Funding programme
Excutive institution
Institute for Epidemiology and Pathogen Diagnostics (JKI-EP)
Funding institution
Project management agencies
Associated projects: STEP-4-STEP
- Collaborative project: Molecular charcterization of different TRV isolates and analysis of the interaction between virus, nematode and potato to improve resistance breeding. Subproject 2
- Collaborative project: Molecular charcterization of different TRV isolates and analysis of the interaction between virus, nematode and potato to improve resistance breeding. Subproject 1
- Collaborative project: Molecular charcterization of different TRV isolates and analysis of the interaction between virus, nematode and potato to improve resistance breeding. Subproject 3
