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Collaborative project: Development of innovative analytical technologies as a prerequisite for the production of safe and high-quality crustacean products and a sustainable stock management - subproject 1 (KrustInUVa)

Project


Project code: 281A104416, MRI-MF-08-174 - 923 1050 KrustInUVa
Contract period: 01.09.2018 - 31.08.2021
Budget: 260,566 Euro
Purpose of research: Experimental development
Keywords: food safety, quality control, allergens, traceability, other aquatic organisms, allergy/incompatibility response, aquatic genetic resources, health-related consumer protection

The project KrustInUVa aimed to develop different DNA- and protein-based methods for species authentication of crustaceans. A comprehensive collection of crustacean species forms the basis for these research activities. Their valid DNA sequences will be published in public databases. To enable a rapid species identification and high throughput of samples, a multiplex real-time PCR for market-relevant crustacean species and a MALDI-TOF method, extended by a database of crustacean mass spectra, have been established. Two meta-barcoding methods have been successfully used for the examination of crustacean mixtures. LC-MS/MS technologies are common in analytical laboratories and can now be used for species authentication of selected crustaceans with the identified species-specific peptides. For consumer health protection reason, an LC-MS method for the detection of allergen-specific peptides in crustaceans has also been developed.

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