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Biodiversity and functional genomics of endogenous small RNAs induced upon biotic and abiotic stresses in plants
Project
Project code: 0313157
Contract period: 01.09.2004
- 31.05.2007
Budget: 159,316 Euro
Purpose of research: Basic research
The aim of the proposed work is to study plant small RNAs through the development of advanced genomic tools. The present state of knowledge on the functions of endogenous small RNAs in plants is limited, and many questions remain unresolved, in particular the possible implication of these molecules on gene regulation in response to abiotic and biotic stress conditions. Our integrated strategy will use both experimental and bioinformatic approaches to address the functional genomics of small RNAs and micro RNAs in plants. This will be conducted by the elaboration of normal growth and stress-specific small RNA libraries in Arabidopsis and tomato. The information generated will be used in the search for candidate genes that are possibly regulated by small RNAs, and in the development of computer-based algorithms for prediction of small RNA precursors as well as stress-responsive elements in their promoters. Following experimental validation of the most relevant results, we propose the generation of a micro-array containing plant small RNAs that will be used to extend our study to different plant species. Comparative genomics approaches will give broad biodiversity dimension to the work through the identification of conserved stress-related small RNAs and possible specific adaptations to stress by diversification.
Section overview
Subjects
- Plant Breeding
Framework programme
BMBF - Frameworkprogramm Biotechnology - Using and Shaping its Opportunities
