We use cookies on our website. Some are necessary for the operation of the website. You can also allow cookies for statistical purposes. You can adjust the data protection settings or agree to all cookies directly.
Data Protection Declaration
Imprint
Differentiation of Mycobacterium avium subsp. paratuberculosis isolates of different origin by a new standardised typing protocol
Project
Project code: FLI-IMP-08-N-0001
Contract period: 01.07.2007
- 30.06.2013
Purpose of research: Experimental development
A novel standardized typing algorithm will be established for Mycobacterium avium ssp. paratuberculosis (MAP) involving a combination of different DNA typing methods to enhance the discriminatory power. MAP isolates recovered from human Crohn?s disease (CD) and bovine Johne?s disease (JD) tissues as well as milk will be typed by this algorithm in order to elucidate possible transmission routes of MAP from cattle to man. Furthermore, investigation of the genetic relationship between MAP isolates of human and different animal origins will provide more detailed information on the existence and possible sources of human disease-associated genotypes of MAP. This will give further clarification regarding the zoonotic role of MAP, which is still under discussion.
Suspicion of Mycobacterium avium subsp. paratuberculosis Transmission between Cattle and Wild-Living Red Deer (Cervus elaphus) by Multitarget Genotyping Isabel Fritsch, Gabriele Luyven, Heike Köhler, Walburga Lutz, and Petra Möbius Multitarget genotyping of the etiologic agent Mycobacterium avium subsp. paratuberculosis is necessary for epidemiological tracing of paratuberculosis (Johne’s disease). The study was undertaken to assess the informative value of different typing techniques and individual genome markers by investigation of M. avium subsp. paratuberculosis transmission between wild-living red deer and farmed cattle with known shared habitats. Fifty-three M. avium subsp. paratuberculosis type II isolates were differentiated by short sequence repeat analysis (SSR; 4 loci), mycobacterial interspersed repetitive-unit–variable-number tandemrepeat analysis (MIRU-VNTR; 8 loci), and restriction fragment length polymorphism analysis based on IS900 (IS900-RFLP) using BstEII and PstI digestion. Isolates originated from free-living red deer (Cervus elaphus) from Eifel National Park (n_13), six cattle herds living in the area of this park (n_23), and five cattle herds without any contact with these red deer (n_17). Data based on individual herds and genotypes verified that SSR G2 repeats did not exhibit sufficient stability for epidemiological studies. Two common SSR profiles (without G2 repeats), nine MIRU-VNTR patterns, and nine IS900-RFLP patterns were detected, resulting in 17 genotypes when combined. A high genetic variability was found for red deer and cattle isolates within and outside Eifel National Park, but it was revealed only by combination of different typing techniques. Results imply that within this restricted area, wild-living and farmed animals maintain a reservoir for specific M. avium subsp. paratuberculosis genotypes. No host relation of genotypes was obtained. Results suggested that four genotypes had been transmitted between and within species and that one genotype had been transmitted between cattle herds only. Use of multitarget genotyping for M. avium subsp. paratuberculosis type II strains and sufficiently stable genetic markers is essential for reliable interpretations of epidemiological studies on paratuberculosis. Appl. Environ. Microbiol. 2012, 78(4):1132. DOI: 10.1128/AEM.06812-11.
Section overview
Subjects
- Animal health
- Biotechnology
- Toxicology
