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Occurrence and spread of quarantine pathogen candidatus phytoplasma ulmi in Elm types in Germany (PhytoUlmus)

Project


Project code: 22026316
Contract period: 01.10.2017 - 30.09.2020
Purpose of research: Inventory & Assessment

Candidatus phytoplasma ulmi is a bacterial intracellular parasite that leads in many areas of yellowing and the death of elms. This important Quarantäneschaderreger has been demonstrated in recent decades in the European Union and in Germany. Germany was occurrence in U. glabra and U. laevis already occasionally documented, while so far no proof at U. minor. The own work to U. laevis may fear that the pathogen in Brandenburg is already regionally established. Overall the available data allow so far neither a statement to the occurrence or regional distribution of the pathogen in the different kinds of elms in Germany. The proposed project is to fill these knowledge gaps titled "Occurrence and spread of quarantine pathogen candidatus phytoplasma ulmi in Elm types in Germany" and the available methods for determination and differentiation of the pathogen significantly improve. The project is aiming at the detection of the pathogen in the context of extensive sampling in selected areas of occurrence or different regions of Germany in the native Elm species. In the project, the molecular diagnosis significantly improved through the development of new probes into the quantitative PCR (TaqMan) and a differentiation reached approx. p. ulmi tribes through the use of new genetic markers. Incidence and epidemiology of the pathogen can be so determined. New protocols for efficient diagnosis and reference material will be created. The results of this project deliver the much needed missing scientific data basis for an assessment of the situation in Germany and providing decision support to the introduction of phytosanitary measures to the Minimizing risk.

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