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Collaborative project: Method developments for the assessment of formulation impact on the activation of dendritic cells in coculture with keratinocytes - subproject 3 (KOEXPOSENS)
Project
Project code: 281A307C18
Contract period: 01.01.2020
- 31.12.2022
Budget: 74,897 Euro
Purpose of research: Applied research
Keywords: allergens, allergy/incompatibility response, articles of daily use, health-related consumer protection, ingredients, cosmetics, product safety, resource protection, resource efficiency
The aim of the present project is to investigate possible interactions between sensitizing fragrance molecules, other cosmetic ingredients as well as hair dye precursors for hair colorations commonly found in consumer products. This serves to improve the assessment of interactions between individual substances or of individual substances with matrices. The project will therefore identify factors that may complement and improve the current approaches for hazard and potency assessment. The endpoint for the assessment is the activation of dendritic cells in a skin-like environment (combination with keratinocytes), known to significantly influence the quality and intensity of antigen-specific T cell activation. This will be achieved by using different keratinocytes (HaCaT keratinocytes, pre-activated keratinocytes and epidermal skin models) in coculture with THP-1 cells as a model for dendritic cells. For example, fragrances, hair dyes and other cosmetic ingredients as well as individual formulations and mixtures thereof are added to cocultured cells or are applied to a skin model (epidermis model) with THP-1 cells placed on the basolateral side. In addition to the investigation of viability and activation state of the THP-1 cells, studies on changes in the release of cytokines and chemokines as well as on the fate or specific conversion of individual substances after exposure are carried out using mass spectrometric methods. The work will provide a test strategy tailored to answer the questions raised. Specifically, the results obtained for the materials tested will provide insights whether the potency of contact allergens can be influenced by other substances and, if so, whether this is specific to particular groups of chemicals. Overall, the results obtained will help a) to determine the extent of interactions, b) to perform their toxicological assessment and c) to consider consequences relevant for the safety assessment.
Section overview
Subjects
- Process engineering
- Toxicology
Framework programme
Funding programme
Excutive institution
Funding institution
Project management agencies
Associated projects: KOEXPOSENS
- Collaborative project: Method developments for the assessment of formulation impact on the activation of dendritic cells in coculture with keratinocytes - subproject 2
- Collaborative project: Method developments for the assessment of formulation impact on the activation of dendritic cells in coculture with keratinocytes - subproject 1
