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Imprint
Collaborative project: Development and validation of a new 3D immune surrogate full-thickness test system for the predictive characterization and identification of the sensitization potential of fragrances and preservatives in consumer products - subproject 3 (3D-ImmunoSkin)
Project
Project code: 281A308C18
Contract period: 01.03.2020
- 31.08.2023
Budget: 222,570 Euro
Purpose of research: Applied research
Keywords: health-related consumer protection, allergy, incompatibility response, allergens, resource protection, resource efficiency, ingredients, product safety, cosmetics, articles of daily use
The proposal presented here is intended to overcome technical, mechanistic and biological limitations of previous in vitro skin test methods. The human skin model "Phenion Full Thickness Skin Model" (Henkel AG & Co KGaA), which is already successfully established on the market and consists of the "scaffold cells" of the skin with only limited immune competence, is to be further completed immunologically in this project and thus considerably upgraded. This skin model has already been validated for genotoxicological evaluations and has found access to corresponding OECD working groups. It is therefore to be expected that the model can also be accepted by regulatory authorities for further use in skin sensitization assessment. We plan to develop cell culture conditions under which surrogates of the Langerhans cells are functionally integrated as central initiator cells of an immune reaction together with fibroblasts and keratinocytes in the tissue of the skin model, in order to map skin sensitization in the skin model. A validated 3D skin model does not yet exist, which also contains immune cells and can at least partially simulate functions of the immune system. Therefore, after integration of monocytic cell lines whose immunological functions can simulate those of Langerhans cells in the epidermis, the functionality of the immune cells as well as of keratinocytes and fibroblasts must be carefully characterized. Various techniques from cell biology are used (vitality and apoptosis assays, analysis of secreted cytokines by flow cytometry, ELISA, and multiplex analyses, flow cytometric expression analyses of surface molecules, stimulation capacity against other immune cells, transcriptome analyses and others). Through the installation of primary immune cells isolated from the patient, a close interaction between in vitro research and clinic can guarantee a particularly efficient and patient-oriented procedure.
Section overview
Subjects
- Biotechnology
- Toxicology
Framework programme
Funding programme
Excutive institution
Funding institution
Project management agencies
Associated projects: 3D-ImmunoSkin
- Collaborative project: Development and validation of a new 3D immune surrogate full-thickness test system for the predictive characterization and identification of the sensitization potential of fragrances and preservatives in consumer products - subproject 1
- Collaborative project: Development and validation of a new 3D immune surrogate full-thickness test system for the predictive characterization and identification of the sensitization potential of fragrances and preservatives in consumer products - subproject 2
