We use cookies on our website. Some are necessary for the operation of the website. You can also allow cookies for statistical purposes. You can adjust the data protection settings or agree to all cookies directly.
Data Protection Declaration
Imprint
Identification, modification and deployment of genetic factors controlling resistance to important pathogensin barley - project part D (IdeMoDeResBar II)
Project
Project code: 031B0887D
Contract period: 01.04.2020
- 31.01.2023
Budget: 287,981 Euro
Purpose of research: Applied research
Keywords: resistance breeding, barley, yellow mosaic virus disease, BaMMV/BaYMV, leaf rust, Puccinia hordei, scald, Rhynchosporium commune
IdeMoDeResBar aims on sustainable improvement of barley yield stability and grain quality to ensure aliment and feed production. Effective resistance genes against agronomically relevant fungal and viral barley leaf diseases shall be identified, characterized in their function and transferred to breeding by providing informative markers for efficient selection. Extension of IdeMoDeResBar continues investigation of resistance genes against barley yellow mosaic virus disease (BaMMV/BaYMV, rym13, rym15), leaf rust (Puccinia hordei, RphMBR1012), and scald (Rhynchosporium commune, Rrs1) towards physical mapping, functional characterization and development of markers for selection. Physical mapping applies chromosome flow sorting and next generation sequencing (NGS) approaches as well as state of the art bioinformatics pipelines. Genetic diversity at the resistance loci is investigated by resequencing of candidate genes in selected genebank and breeding accessions. Functional analysis of resistance genes comprises expression analysis of candidate genes during infection, microscopic analysis of host-pathogen-interaction as well as validation by CRISPR/Cas9- driven genome editing or overexpression. Based on these investigations IdeMoDeResBar-II provides markers for fast and low-cost breeding in particular for small breeding companies i) to improve the genetic diversity of resistance and ii) to accelerate the transfer of up to date unknown alleles of needed resistance genes to elite barley and are crucial for sustainable agriculture.
Section overview
Subjects
- Crop Production
- Plant Breeding
