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Imprint
Development of a signal-enhanced immuno-capture amplification for the rapid detection of the quarantine pathogen Candidatus Phytoplasma vitis and the regulated non-quarantine pathogens Candidatus Phytoplasma mali and Candidatus Phytoplasma pyri (PhytoSica)
Project
Project code: 2818708X19
Contract period: 01.07.2021
- 31.08.2024
Budget: 294,660 Euro
Purpose of research: Experimental development
Keywords: vine, quarantine pathogens, plant diseases (virusus, bacteria, fungi, phytoplasma), crop protection, plant health, diagnostics, pome
Within the project PhytoSica it is intended to develop a rapid detection method based on immunological and molecular procedures for three important European fruit phytoplasmas, namely for the quarantine pathogen Candidatus Phytoplasma vitis and for the regulated non-quarantine pathogens Candidatus Phytoplasma mali and Candidatus Phytoplasma pyri. For each pathogen a specific monoclonal antibody will be developed which is directed against the frequently occurring membrane protein IMP, or VMP for Ca. P. vitis. The monoclonal antibodies are linked to reaction vial surfaces and will bind phytoplasma cells from plant extracts. Subsequently, the same antibody, now conjugated with DNA-fragments, is added and will bind to free epitopes. In the following step the conjugated DNA-fragment will serve as the target for the isothermal DNA-amplification by a recombinase. The enzyme will incorporate modified primers which can be visualized colorimetrically on a dipstick after the reaction. The whole procedure is performed with simple devices and suited for a point-of-care application.
Section overview
Subjects
- Crop Protection
- Viticulture
Framework programme
Funding programme
Excutive institution
Institute for Plant Protection in Fruit Crops and Viticulture - Viticulture (JKI-OW)
