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Imprint
Adding layers of protection to gain a resistant status against M. chitwoodi, PLRV, PVY and TRV (ADLATUS)
Project
Project code: JKI-EP-08-2354
Contract period: 01.12.2020
- 31.01.2024
Purpose of research: Applied research
The goal of this joint project is the improvement of existing, as well as establishment of novel defence mechanisms against diverse pests. Meloidogyne chitwoodi, a quarantine-status endoparasitic root knot nematode is one of the major pests of potatoes. PVY (potato virus Y) and PLRV (potato leaf roll virus) are the regulated non-quarantine viruses, which are transmitted by the aphids, e.g. Myzus persicae. The tendency for the reduction of pesticide use will result in a significant increase in the occurrence of PLRV, as aphids that have taken up PLRV-virions stay viruliferous for their entire life. In the potato germplasm there is a single gene originating from S. stoloniferum, which results in PVY immunity, which is, however, linked with male sterility. Therefore, new and complementary resistance sources need to be identified for both viruses. The tobacco rattle virus (TRV) is transmitted by ectoparasitic nematodes belonging to the genera Trichodorus and Paratrichodorus. As currently available nematicides show only limited control against these viral vectors, the efficient resistances originating from wild potato species need to be identified. Additionally, the project aims at development of novel levels of pathogen resistance. The efficient replication of PVY depends on successful hijacking of host translation mechanisms. Therefore, the inactivation of the translation initiation factor (eIF4e) results in the inhibition of viral replication. The efficiency of this approach against infection by PLRV and TRV will be tested as well. Following the damage to sieve elements in plants, sieve plates can be closed either temporarily by phloem proteins (P-proteins), or permanently by callose deposition. Inactivation of genes responsible for P-protein synthesis will result in permanent closure of sieve plates. This may prevent flow of nutrients towards the feeding site induced by M. chitwoodi, resulting in parasite starvation and leading to increased resistance.
Section overview
Subjects
- Crop Production
- Crop Protection
Framework programme
Funding programme
Excutive institution
Institute for Epidemiology and Pathogen Diagnostics (JKI-EP)
