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Development and testing of practicable procedures for inactivation and detection of potato wart disease causing fungus Synchytrium endobioticum in solid and liquid soil) residues (SYNergie) (SYNergie)
Project
Project code: JKI-A-08-1312, 28A8705A19
Contract period: 15.06.2021
- 14.08.2024
Purpose of research: Applied research
The potato wart disease causing fungus Synchytrium endobioticum is considered one of the three most significant pests of potato plants due to severe harvest losses and missing eradication measures. Sori (winter sporangia, resting spores) of the fungus are extremely robust and - according to recent results - may survive in the field for up to 46 years. During processing of potatoes, some hundreds of thousands of tons of solid and liquid residual material accumulate each year via tuber-attached soil and remnants from potato treatment (damaged tubers, potato peel, washing water). The aim of this proposal is to develop and test procedures for effective and cost-efficient inactivation of sori in residual material which will allow to constrain the distribution of the pest and to return the soil to the fields. Among the procedures to be tested for liquid waste are chlorine dioxide, ultrasound, pulsed-electric-field technology, UV-C irradiation and for solid waste intermixing of burnt lime, ohmic heating as well as rotary drying. Furthermore, combinations of different procedures including chemical and/or enzymatical pre-treatment shall be evaluated. Based on these findings approaches for the implementation of effective sanitation procedures will be established for the potato-processing industry in order to determine the requirements that the procedures request from the companies‘ operational level. In parallel, methods shall be investigated that enable determination of sorus viability as a safe and effective alternative to the conventional biotest for proving the success of treatments. In this context, new techniques will be tested including confocal laser scanning microscopy (CLSM), miniaturized biotests, intron-based PCR of RNA and Raman spectroscopy. Analytical and sensoric methods shall be established to determine differences in VOC pattern of infected and non-infected plants via GC-MS-based technology.
Section overview
Subjects
- Crop Production
- Crop Protection
- Soil science
- Resource management
Framework programme
Funding programme
Excutive institution
Institute for Plant Protection in Field Crops and Grassland (JKI-A)
