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Imprint
Creating a catalog of fish (at the database level) with all available analytical data on the molecular biology of fish Differentiation
Project
Project code: 03HS010
Contract period: 01.10.2004
- 31.03.2005
Budget: 32,377 Euro
Purpose of research: Applied research
Fishery products, according to the regulation (EU) 104/2000 of the European Council from the 17th of december 1999, need to be clearly labled concerning to
Trade name of fish-, crustacean- and mollusc species Method of production (marine fishing, freshwater fishing and aquaculture)
Catching area (e.g. Northeast-Pacific, North Sea)
Regarding this, a catalogue to verify the labelling of fishery products has been generated. Gain of the project was to make data such as
IEF (Isoelectric focusing)
SSCP DNA single strand conformational polymorphism
PCR-RFLP Polymerase Chain Reaction Restriction Fragment Length Polymorphism
DNA-Sequencing sequence of a defined sector for determining species
Alignment Comparison of DNA sequences
available. Main focus was set on fish species with a high economic relevance.
The conceptual formulation was to sample all relevant analytic data, to choose an appropriate database programme and to enter all available data into the database.
Method
The compilation of the database as well as the selection of adequate software and the programming procedure was accomplished by the company Planomedia. The database is based on different Open Source components. The data processor which is used by an operator merely needs a web browser for working with the database.
The refurbishment of DNA sequences of marine organisms such as fishes, crustaceans and molluscs was undertaken by the company Impetus GmbH & Co. Bioscience KG.
Results
The database is clearly structured and easy to handle. The user has the facility to search after a particular fish, mollusc or crustacean by entering the german or scientific name. Another way is to go through a list of all available species if the correct name is not known. At present, the database contains analytic data from 198 fishes, 14 crustaceans and 19 molluscs. The complete data set including IEF, SSCP, RFLP, DNA and alignment is merely only available for a few species. The requirement for future users will be to complete the lacking data sets as well as to enter new analytic data of marine organisms if possible.
Available to most of the species is a short summary including distribution and habitat requirements. The molecular biological methods are described as well on therefore provided pages.
Conclusion
The aim, to design a database for analytic information of marine organisms with high economic relevance and to enter such information into the database has been reached in therefore mentioned period of time.
For further steps, the responsibility for this product will be referred to staff members of the Bundesforschungsanstalt für Ernährung und Lebensmittel (BFEL), FB Fischqualität.
All data can be used as comparison or standards for definite identification and determination of species regarding to their trade name.
Section overview
Subjects
- Freshwater fisheries
- Marine fisheries
- Raceways systems
- Recirculation systems
- Mariculture
Framework programme
Funding programme
Excutive institution
Department of Naval Architecture and Marine Engineering, Nautical Science, Biology, Bionics
