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Collaborative project: Breeding of pear varieties resistant to Pear decline (BISO) – Subproject 1

Project


Project code: 2814501610
Contract period: 01.03.2011 - 28.02.2014
Budget: 156,336 Euro
Purpose of research: Experimental development

Pear decline which is a disease caused by Candidatus Phytoplasma pyri threatens commercial and private pear growing in Germany. In close cooperation with the private fruit breeding enterprise Bayerisches Obstzentrum, the institute of fruit science of Technische Universität München developed an easy to handle and robust system for the selection of resistance to pear decline. This system enables the screening of existing and future breeding populations. The nucleic acid-based method LAMP (loop associated isothermal amplification) was adapted to pear decline detection and combined with a color test. The evolved primer set is also able to detect Candidatus Phytoplasma mali causing apple proliferation disease. This molecular testsytem is now available for breeders and can be used for phytoplasm detection during the selection process of resistant clones using test graftings. This will reduce the time period of breeding cycles. The method is applicable for the health control of plant material used for propagation such as bud sticks or rootstocks.

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Subjects

Excutive institution

Department for Pomiculture

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