Plant biotechnology of the future - A genomics assisted approach to tap the potential of the secondary gene pool for sustainable barley breeding (TRANS-BULB) - Subproject C | FISA - Forschungsinformationssystem Agrar und Ernährung

Plant biotechnology of the future - A genomics assisted approach to tap the potential of the secondary gene pool for sustainable barley breeding (TRANS-BULB) - Subproject C

Project

Production processes

This project contributes to the research aim 'Production processes'. Which funding institutions are active for this aim? What are the sub-aims? Take a look:
Production processes

Project code: 0315966C
Contract period: 01.09.2012 - 30.08.2015
Budget: 143,414 Euro
Purpose of research: Applied research

In barley breeding, the wild species Hordeum bulbosum (Hb) has the potential to serve as resource of novel variability in valuable traits which so far has only scarcely been used. A number of novel barley-disease resistances have been introgressed from H. bulbosum to cultivated barley (Hordeum vulgare ssp. Vulgare, Hv) via interspecific crosses, among them dominantly inherited resistances to the BaMMV/BaYMV-1/-2 virus complex (resistance genes Rym14Hb, Rym16Hb), BYDV (resistance gene Ryd4Hb), scald (Rrs16Hb), leaf rust (Rph22Hb) as well as resistance to cereal cyst nematodes (inheritance yet to be characterised). Introgression lines carrying either of these resistances are available and some of these have tentatively been used in barley breeding programmes, demonstrating that Hb-derived disease resistances may be valuable to barley breeding. Given this Proof of Concept, efficient use of this novel resistance resource in barley breeding is, however, hampered by the limited number of Hb-specific molecular markers flanking the respective Hb target gene. Such markers are needed to break down the fairly large sizes (and partly, linkage drags) of initial Hb introgressions in a directed approach relying on meiotic recombination, i. e., via marker-assisted back¬cross breeding followed by the generation of double-haploid lines (DHL). Ziel des Verbundprojekts TransBulb ist die Hochdurchsatzentwicklung von Molekularen Markern für chromosomale Introgressionen aus Hordeum bulbosum in Hordeum vulgare. Mit neuen molekularen Markern soll eine präzise genetische Charakterisierung der Introgressionen erreicht werden. Neue Marker dienen dazu, die Verkleinerung von Introgressionen aus H. bulbosum durch Rekombination dokumentieren und gezielt selektieren zu können, um negativen linkage drag, der an die Introgressionen gekoppelt ist, zu minimieren. Neue diagnostische, eng-gekoppelte Marker sollen von den kooperierenden Züchtungsunternehmen für den eigenen marker-gestützten Selektionsprozess eingesetzt werden können. Die geplanten Arbeiten bauen auf die am JKI entwickelten pflanzengenetischen Ressourcen sowie auf die genomischen Ressourcen für Gerste des Projektes GABI-BARLEX auf.

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