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Data Protection Declaration
Imprint
Development of new methods for the identification of animal proteins in feedingstuff. Real-time immuno-PCR for the detection of animal bone and tissue marker 2010: Validation of an immunological method for the detection of Osteocalcin representing a stable bone tissue marker in Meat and Bone Meal (MBM)
Project
Project code: BfR-LMS-08-1322-321
Contract period: 01.04.2008
- 31.12.2010
Purpose of research: Applied research
Feeding of animal protein to farmed animals is - with just a few exceptions - not allowed according to Regulation (EC) No. 999/2001. The official method for the detection of animal particles in feed is based on microscopy Regulation (EC) No. 152/2009). However, microscopy cannot distinguish animal species. Further quantitative analysis poses a problem as demonstrated in ring trials organised by the Community Reference Laboratory for Animal Proteins (CRL-AP) in Gembloux . Thus, at present there is an intensive search for alternative methods. The verification of the analyte 'animal protein' by an immunological based method unifies the advantage of usually high sensitivity with a relatively simple technique without the need for special equipment. Moreover the development of rapid tests (e.g. dip-stick-format, lateral flow device) is possible. But so far all commercial kits intended for the detection of animal species in feed which have been validated by order of the European DG-SANCO via CRL-AP, revealed certain deficiencies. The test kits which in most cases are build up on the heat stable muscle protein Troponin C, partly also on collageneous material, could not meet the requirements of robust methods concerning specificity and/or sensitivity so far. Above all cross reaction of the antibodies with different feed components like e.g. citruspulp, sugar beat, but also rendered pig fat, was observed. In the same way the claim for differentiation of ruminant protein from other animal species like pig ? for example in a huge background of the other animal species ? could not be supported by the present vali-dation studies because of lacking selectivity. A new marketed test (MELISA-TEK, Elisa Technologies Inc., Gainesville, FL, USA), which should differentiate Troponin I of ruminants from swine, seems not to give satisfying results (Pascal Veys, CRL-AP, pers. comm.).
Section overview
Subjects
- Animal nutrition
- Biotechnology
