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Evaluation of Winter barley varieties and breeding lines with resistance to loose smut (Ustilago nuda) for susceptibility to natural infection with barley leaf stripe (Drechslera graminea)

Project


Project code: 2810OE073
Contract period: 01.09.2011 - 31.12.2014
Budget: 139,712 Euro
Purpose of research: Applied research
Keywords: winter barley, barley leaf stripe, resistance screening

'Winter barley varieties presently available on the German and Austrian market and advanced breeding lines from 'Forschung & Züchtung Dottenfelderhof' are going to be analysed for resistance to barley leaf stripe, a fungal disease caused by Pyrenophora graminea (Ito et Kurib.) [Drechslera graminea (Rabenh. ex. Schlech.) Shoemaker] under the conditions of organic farming. The project will be conducted over three vegetation periods (2011/12, 2012/13 and 2013/14). In the first vegetation period the varieties will be sown only on the site 'Dottenfelderhof', Bad Vilbel because this location turned out to be highly efficient for natural infection in former tests (Müller 2006). The varieties will be subjected to natural inoculum by placing them adjacent to highly susceptible lines (spreader rows). In the second vegetation period the varieties will be harvested and sown on the test sites 'Dottenfelderhof' and 'Darzau' to evaluate the infection under different local conditions. This will simulate the conditions of seed propagation and increase the infection pressure in the last testing year. In the third vegetation period 2013/14 the varieties for evaluation will be sown without spreader rows on both test sites.'

The project "Evaluation of Winter barley varieties and breeding lines with resistance to loose smut (Ustilago nuda) for susceptibility to natural infection with barley leaf stripe (Drechslera graminea)" started in autumn 2011 and was conducted until end of 2018. During the project 244 accessions were evaluated for stripe disease at least once. The level of infestation was rising continuously during the project and in 2017 it reached 48 % for the most susceptible accession. Only three accessions were found completely disease free. The majority of accessions showed a disease level between 1 % to 5 %.
Three breeding lines of 'Research & Breeding Dottenfelderhof' that were tested for at least three years, were found with a maximum stripe disease level Different laboratory methods for the determination of stripe disease on the seeds were tested. Until the end of the project no satisfactory correlation between field infection and laboratory results could be found.

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