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Investigation of interactions between soil-borne sugar beet viruses and their effect on the rhizomania development
Project
Project code: JKI-EP-08-2208
Contract period: 01.07.2011
- 31.12.2015
Purpose of research: Basic research
In the previous research project the occurrence of soil-borne viruses in the most important German producing areas of sugar beet was analysed. The results showed, that in 62% of regions infested with soil-borne viruses infections are caused by a mix of Beet necrotic yellow vein virus (BNYVV), Beet soil-borne virus and Beet virus Q. The virus content
of soil samples was determined by growing sugar beet seedlings in these soils as bait plants under controlled conditions in a climate chamber. Furthermore, methods have been developed to separate the different viruses starting from mixed-infected sugar beet plants and us Chenopodium quinoa and C. rubrum as differential hosts. Pure isolates were
established and maintained. New PCR variants for the quick and economical simultaneous detection BNYVV, BSBV, BVQ and two other soil-borne pathogens endangering sugar beet outside Europe Beet soil-borne mosaic virus (BSBMV) and Beet black scorch virus (BBSV). The fungal virus vector, Polymyxa betae Keskin, was loaded by vortex inoculation with soil-borne viruses in different combinations. Taken together, the basis for the further investigation of the interactions between soil-borne viruses in mix-infected plants was established. The aims of the current research project are the elucidation of interactions between sugar beet soil-borne viruses, the detection of their effects on the rhizomania disease and on the sustainability of resistances against BNYVV. To characterize pathogen relevant interactions it is necessary to produce selected virus-vector-populations with a high efficiency of virus transmission, to investigate the dissemination of each virus in different host plant genotypes and to monitor the plant growth under different infection conditions and pathogen burden.
Section overview
Subjects
- Crop Protection
Framework programme
Funding programme
Excutive institution
Institute for Epidemiology and Pathogen Diagnostics (JKI-EP)
