We use cookies on our website. Some are necessary for the operation of the website. You can also allow cookies for statistical purposes. You can adjust the data protection settings or agree to all cookies directly.
Data Protection Declaration
Imprint
Modern molecular diagnostic methods: Development and use of the DNA chip technology for comprehensive influenza virus diagnostics
Project
Project code: 2813100106
Contract period: 01.07.2006
- 30.06.2009
Budget: 469,294 Euro
Purpose of research: Experimental development
For the rapid detection of infections with AIV (especially HPAI H5N1) and for a detailed diagnostic characterisation of the pathogen, the use of modern molecular diagnostic methods is vital. The real time PCR which is already in large-scale use at the FLI has significant advantages for routine diagnostics. However, for a rapid and detailed further characterisation of virus isolates, the use and improvement of additional molecular diagnostic methods are indispensable. For the ad hoc analysis of different AIV isolates, the DNA chip technology is especially suitable, as it permits to analyse a multitude of different genome sections in parallel. For veterinary influenza diagnostics, the development and use of DNA chip systems are foreseen for the following areas: a)Characterisation of HA and NA subtypes. We plan to establish systems which in addition to subtyping of H5 and H7 permit the rapid identification of all currently existing HA subtypes (H1 to H16) and NA subtypes (N1 to N9). Isolates from different species, including wild birds, can thus be classified directly into a specific subtype b)Investigation of antigen modifications in isolates of a current outbreak. Currently occurring H5N1 isolates can thus be classified into specific genotypes, and genetic elements which are a sign for an adaptation of a current avian influenza A virus to other hosts (e.g. humans) can rapidly be detected. In addition to an assessment of the occurrence of different subtypes in domestic and wild bird populations, the occurrence of variants and new subtypes can be diagnosed at a very early stage c)Use of DNA chip technology for analysis of virulence markers. In addition to identifying the cleavage site sequence of the HA gene, we also plan to analyse other potential virulence factors of the individual isolates. Thus, point mutations in the nonstructural proteins as well as special sequences in gene segments with important pathogenetic properties (e.g. NS1) can be differentiated d)Parallel differential diagnostic identification of other pathogens which are of importance in veterinary medicine, such as e.g. an infection with Newcastle Disease virus (“atypical fowl plague”). Infections which can simulate an AIV infection can thus be excluded safely and rapidly. Furthermore, DNA chip systems permit the identification and characterisation of influenza A viruses in other animal species (e.g. pigs and horses).
Section overview
Subjects
- Animal health
- Special animal species
