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Optimisation of conventional AIV diagnostics: AIV detection: sensitive recombinant cell culture systems
Project
Project code: 2813101506
Contract period: 01.01.2007
- 31.12.2009
Budget: 343,654 Euro
Purpose of research: Experimental development
Work with influenza viruses is significantly hampered by the fact that low pathogenic subtypes cannot be replicated continuously in cell culture. Virus isolation and mass replication (vaccine production) must be done in embryonated chicken eggs, which is expensive and time-consuming. Using the archive of cell lines at the FLI, the principal permissivity of different cell cultures from pig, duck and swan will be investigated. Cells with a basic permissivity will be provided with a secernable trypsinergic protease (e.g. human airway-specific protease, enteropeptidase, mast cell tryptase). Following analysis of the sialoreceptors of the cell line, the missing sialotransferase will be added if necessary. In addition, procedures for the endogenic inhibition of the interferon expression will be investigated (e.g. RNAi technology, co-expression of pestiviral Npro). The resulting cell lines should replace the use of embryonated chicken eggs in diagnostics (virus replication, neutralisation tests).
Section overview
Subjects
- Animal health
- Biotechnology
