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Tilletia - Molecular biological differentiation and quantification
Project
Project code: 151
Contract period: 01.09.2011
- 31.12.2013
Purpose of research: Inventory & Assessment
Common bunt (Tilletia caries) and dwarf bunt (T. controversa) are important phytopathogens and mycotoxin producers in cereals and grasses. Laboratory analyses make sure that the spores of these fungi do not spread through seeds and that feeds are not contaminated with spores or mycotoxins such as trimethylamine which is a threat to human and animal health. Various classical detection methods with haemocytometer (ISTA method) or filtration (LTZ method) are time-consuming and have problems with quantification. In addition, the morphological differentiation with the microscope is not always possible beyond doubt. Thus, a new PCR method was established to discriminate and to quantify both species directly from the sample.
Section overview
Subjects
- Animal nutrition
- Food microbiology
- Toxicology
