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Development of molecular biological methods to qualtitativen and quantitative detection and for differentiation of Fusarium spp.
Project
Project code: keine Angaben
Contract period: 01.09.2003
- 31.12.2007
Purpose of research: Applied research
In recent years, ear-fusariosis has increased particularly in wheat. These are in two respects a problem: First, they reduce the yield, on the other hand, the Be-case through the formation of so-called mycotoxins (trichothecenes) leads to a significant deterioration in the quality of the wheat. To improve the diagnosis the test should be highly specific and sensitive polymerase chain reaction (PCR)-method for the qualitative detection of the various occurring on wheat Fusarium species are developed. In addition, PCR systems based on the TaqMan ® technology for the quantification of primary inoculums at the beginning of the vegetation period and the quantitative detection of Fusarium infestation during the growing season and harvested in the developed. This basic sizes for one of the LFL planned test decision support model for the minimization of Fusarium attack and toxin exposure during wheat provided.
Section overview
Subjects
- Crop Protection