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Imprint
Evaluation of optimal transport condition for the shipping of human organotypic corneal equivalents for in vitro drug absorption studies
Project
Project code: BfR-ZEBET-08-1328-488
Contract period: 01.12.2011
- 31.12.2014
Purpose of research: Applied research
The purpose of this project was the development of quality maintaining transport conditions for the shipping of cultured corneal equivalents. The possibility of shipping would improve the applicability of the prevalidated cornea model and advance the reduction and replacement of animal experiments.
The investigations have shown that the influence and the interactions of single parameters are complex and not easy to predict. Especially, the forces resulting from movement or changes in position during the shipment have a great impact on the epithelial barrier which is one key factor for quality control. In general, the viability of the human corneal equivalent was more robust towards differing environmental factors than the transepithelial electrical resistance. With the resulting shipping protocol it was finally possible to send the models to another laboratory with a success rate of 21% for pharmacokinetic studies. Because of the higher robustness of viability it can be presumed that the yield of the developed shipping procedure for toxicological studies will be considerably higher. PD Dr. Reichl was awarded the Felix-WankelAnimal-Welfare-Award 2013 for his work.
Section overview
Subjects
- Process engineering
Framework programme
Funding programme
Excutive institution
BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments (BfR - ZEBET)
