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Rapid DNA chip-based test for qualitative fish species differentiation
Project
Project code: AiF 18667 N, MRI-MF-08-151-306-1050 Fish-Chip
Contract period: 01.09.2016
- 31.08.2019
Purpose of research: Applied research
Consumers can nowadays choose among a variety of seafood species from all over the world due to globalized supply chains. There are likely more than 500 fish species available on the German market. The fish industry and fish traders have to guarantee correct labelling of fish products not only with the commercial designation but also with the scientific name of the exact species. If the industry imports already processed fish goods like fish fillets, or fish species which are not easy to distinguish (e.g. some tunas or snappers), a visual identification of the species is almost impossible. Mislabelling of fish products can be detrimental to the companies and cause expansive product recalls, penalties or image loss and leads to distortions of competition. Current analytical techniques for fish species identification are either not specific enough (isoelectric focusing), time-consuming (sequencing of PCR products) or target only one or a few particular species (real-time PCR) and are not suited for on-site application. The aim of the project is to develop a DNA microarray-based method with a fast isothermal DNA amplification step for the identification of the ten most important fish species on the German market and two additional high-priced shrimp species as a proof-of-principle. This method is supposed to be simple to perform, fast, safe from cross-contaminations and to include automated data interpretation in order to be applied on-site, either directly in companies of the fishing industry or in the commissioned commercial laboratories.
Kappel, K., Eschbach, E., Fischer, M., & Fritsche, J. (2019). Design of a user-friendly and rapid DNA microarray assay for the authentication of ten important food fish species. Food Chemistry, 125884. https://doi.org/https://doi.org/10.1016/j.foodchem.2019.125884.
Section overview
Subjects
- Food Chemistry
- Process engineering
Framework programme
Funding programme
Excutive institution
MRI - Department of Safety and Quality of Milk and Fish Products (MRI-MF)
