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Improving blackleg diagnostics and National Reference Laboratory for Blackleg
Project
Project code: FLI-IBIZ-08-DA_0002
Contract period: 01.01.2001
- 31.12.2020
Purpose of research: Applied research
Keywords: Law on epizootic diseases
Blackleg is an acute, infectious, but non-contagious gas oedema with an epizootic course. Young cattle are most commonly affected. However, younger calves, older cattle as well as sheep and goats of any age may become infected as well. Blackleg is a notifiable disease in Germany. The causative agent is Clostridium (C.) chauvoei, which has to be differentiated from other gas edema causing bacteria, especially C. septicum, the infectious agent of malignant edema. Further Clostridium species have to be considered in differential diagnosis. The research is focused on detection and characterization of C. chauvoei.
To improve the molecular diagnostic possibilities in sample material like infected tissue as well as for the identification of isolates a multiplex real-time PCR assay for the simultaneous identification of C. chauvoei and C. septicum was developed. Research topics are the development of further detection and typing methods and the continuous extension of the strain collection. Thomas P, Abdel-Glil MY, Busch A, Wieler LH, Eichhorn I, Bodenthin-Drauschke A, Neubauer H, Seyboldt C (2020) Complete High-Quality Genome Sequence of Clostridium limosum (Hathewaya limosa) Isolate 14S0207, Recovered from a Cow with Suspected Blackleg in Germany. Microbiol Resour Announc 9(2). pii: e01487-19. doi: 10.1128/MRA.01487-19. Thomas P, Semmler T, Eichhorn I, Lübke-Becker A, Werckenthin C, Abdel-Glil MY, Wieler LH, Neubauer H, Seyboldt C (2017) First report of two complete Clostridium chauvoei genome sequences and detailed in silico genome analysis. Infect Genet Evol. 2017 Oct;54:287-298. doi: 10.1016/j.meegid.2017.07.018. Jandowsky A, Bodenthin A, Seyboldt C, Frölich K (2013) Sudden death of outdoor housed pigs caused by Clostridium novyi. A case report. Tierarztl Prax Ausg G Grosstiere Nutztiere 41(6):392-5. German. Seise B, Pollok S, Seyboldt C, Weber K (2013) Dry-reagent-based PCR as a novel tool for the rapid detection of Clostridium spp. J Med Microbiol 62(Pt 10):1588-91. doi: 10.1099/jmm.0.060061-0. Lange M, Neubauer H, Seyboldt C (2010) Development and validation of a multiplex real-time PCR for detection of Clostridium chauvoei and Clostridium septicum. Mol Cell Probes 24(4):204-10. doi: 10.1016/j.mcp.2010.03.003.
Section overview
Subjects
- Animal health
Framework programme
Funding programme
Excutive institution
FLI - Institute for Bacterial Infections and Zoonoses (FLI-IBIZ)
