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Imprint
Immunity suppressive action of Salmonella effector proteins in plant cells
Project
Project code: JKI-EP-08-2292
Contract period: 01.10.2016
- 30.09.2019
Purpose of research: Experimental development
Increasing number of reports, linking food poisoning with the consumption of Salmonella contaminated raw vegetables and fruits, suggest that these bacteria use both animals and plants as potential hosts. Most studies suggested an extracellular lifestyle of Salmonella on plants, however, recent results have shown that Salmonella are able to colonize the intracellular compartments of various plants and actively suppress plant immune system. Salmonella trigger complex host defence responses in Arabidopsis but can overcome the innate immune system to replicate and finally kill the plant. The major aim of this proposal is therefore to identify effector proteins used by Salmonella to counteract the plant immune system. In addition, we aim to identify the targets of those bacterial proteins in plant cells. To achieve this, we will perform a two-step screen for effectors functional in plant cells. These screens will be based on increased virulence of Pseudomonas bacteria expressing Salmonella effectors and on suppression of PAMP-induced signalling in Arabidopsis plants. Chosen effectors will be further analysed for impact on plant immune system and possible targets in plant cells using three independent protein-protein interaction assays. Knowledge gained from this project shall help to compare the human and plant infection systems, which can be used not only in the prevention of human infection via infected vegetables and fruits, but also in better understanding the human infection mechanism itself.
Section overview
Subjects
- Toxicology
Framework programme
Funding programme
Excutive institution
Institute for Epidemiology and Pathogen Diagnostics (JKI-EP)
