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Genome wide association studies to improve drought stress tolerance in Ethiopian wheat (Triticum durum) and barley (Hordeum vulgare) accessions
Project
Project code: JKI-RS-08-3376
Contract period: 01.01.2015
- 31.12.2019
Purpose of research: Applied research
The project aims at identifying drought tolerant wheat and barley genotypes from Ethiopian landrace collections and the identification of genomic regions or genes involved in drought stress response in barley and durum wheat using genome wide association genetics studies. Based on these results easy to handle molecular markers will be developed for combining positive alleles for drought stress which will be used to introgress drought stress tolerance in adapted Ethiopian barley and durum wheat cultivars. 250 barley and 250 wheat (T. durum) genotypes will be selected by the Ethiopian partner and genotyped using the 90k iSelect chip of wheat and the 9k iSelect chip of barley. The 250 wheat and barley genotypes will be phenotyped in Ethiopia and in growth chamber experiments in Germany. Based on these genotypic and respective phenotypic data genome wide association studies will be conducted and respective SNP markers will be converted to easy to handle PCR-based markers. Results obtained within this project will result in detailed information on the genetic variability concerning drought stress tolerance in Ethiopian barley and durum wheat landraces and the identification of genomic regions involved in this tolerance. These results and the respective molecular markers developed will be the base for the development of marker based selection procedures for drought stress tolerance as a prerequisite to increase the growing area and yield stability of barley and durum wheat in Ethiopia. Up to now seed propagation was conducted for 260 barley and durum wheat genotypes and a first trial comprising a well watered and a drought stress variant were sown at two locations in three replications each. After successful propagation of seeds of 260 barley and durum wheat accessions, trials in a drought stress and well water variant in 3 replications each were conducted at two locations. However, due to severe bird damage in the off season, trials could not be harvested. Therefore, during a stay in Ethiopia, the project plan was changed and trials were conducted in the main growing season at two drought stress locations and two well watered locations. Trials were harvested sucessfully and statistical analyses is conducted.
Section overview
Subjects
- Plant Breeding
Framework programme
Funding programme
Excutive institution
Institute of Resistance Research and Stress Tolerance (JKI-RS)
