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Imprint
Comprehensive phenotyping of agronomic traits and six physiological indicators for drought stresstolerance are conducted on a diverse set of rapeseed genotypes
Project
Project code: JKI-RS-08-1135
Contract period: 01.09.2011
- 31.01.2015
Purpose of research: Experimental development
The project aims at estimating the genetic diversity present in oilseed rape on the phenotypic and genotypic level as the base for a directed improvement of yield and yield stability. At JKI respective genotypes will be evaluated for drought tolerance. The project contributes to the characterisation of genetic resources and their use in breeding programs as well as to adapting crops to the changing climate.
Results
Comprehensive phenotyping of agronomic traits and six physiological indicators for drought stress tolerance has been conducted on a diverse set of rapeseed genotypes (PreBreedYield-collection). A two years trial under rain-out shelter conditions and a growth chamber experiment were started and respective genotypes were analyzed in terms of drought stress reaction. In growth-chamber trials induced drought stress conditions were applied on leaf discs and whole leaves whereas in rain-out shelter trials drought stress was applied during flowering. By correlating results obtained in vitro to field data, the traits membrane stability, the concentration of soluble sugars and osmolality were identified suited for high throughput screening.
For the first growth chamber experiment, results for the examined traits indicate significant effects (P=0.001) of induced drought relative to the control treatment and differences between single genotypes under stress conditions were detected. Results of the rain-out shelter trial indicate a significant effect of applied drought stress and show differences between genotypes at a threshold of P=0.001. As an indicator for applied drought stress, the drought intensity value D (FISHER & MAURER, 1978) was calculated over all genotypes and revealed drought intensities of 0.11 for 2012 and 0.49 for 2013, whereas a higher value (0-1) indicates a more severe stress.
Based on results obtained on physiological traits under stress conditions in growth chamber experiments, correlations to yield performance tested in two years were calculated. These correlations are the basis for the selection of best indicator traits for drought tolerance. Overall osmolality, total soluble sugars and membrane stability with coefficients of correlation (SPEARMAN) of 0.33, 0.30 and 0.24 to predict yield performance under drought (0.31, 0.29 and 0.28 for 1000 seed weight) are the major predictors. These indicator traits for drought tolerance; membrane stability, total soluble sugars and in addition over all osmolality are tested on 1064 genotypes in 3 independent replications.
Section overview
Subjects
- Crop Production
- Climate Change
Framework programme
Funding programme
Excutive institution
Institute of Resistance Research and Stress Tolerance (JKI-RS)
