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Collaborative project: Application of bacteriophages for quantitative reduction of Campylobacter in broiler chickens – Subproject 1
Project
Project code: 2816100107
Contract period: 01.02.2008
- 30.04.2011
Budget: 228,117 Euro
Purpose of research: Experimental development
Campylobacteriosis is the most prominent infectious zoonotic disease in Germany. A fast and reliable detection of Campylobacter is necessary in order to identify the microbial risk in the food chain. Beside the detection of Campylobacter it is also important to interfere with the propagation of Campylobacter especially at the side of primary poultry production. A promising option for this is the use of a phage which uses the bacterial cell for propagation and finally destroying it. This project deals with the development of qPCR systems for the quantitative detection of Campylobacter as well as the isolation and identification of phages specific for Campylobacter For the practical and industrial use of these phages qPCR detection systems for the phage were also developed Following results were obtained 1.) Detection of Campylobacter: We developed a quantitative qPCR kit.The kit can be used with different matrices from primary production, slaughter and finally food detailing. The kit is easy to use and is compatible to the majority of the different qPCR cycler brands. For the interpretation of the results no expertise in microbiology is necessary. 2.) A natural Campylobacter-phage was isolated and identified. The phage was successfully tested under real conditions. The use of the phage allowed reducing Campylobacter 1000fold. 3.) For the controlled and further technical use of the phage for Campylobacter reduction a qPCR system for the quantitative detection of the phage was developed. With the help of this kit the phage concentration can be easily adjusted in order to monitor the production and the use of the phage.
Section overview
Subjects
- Animal Husbandry
- Animal health
