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Imprint
Nrf2-deficient human cell lines as a biological tool to screen for compounds of toxicological concern in consumer products.
Project
Project code: BfR-PRS-08-1329-059
Contract period: 01.12.2007
- 31.12.2008
Purpose of research: Basic research
We propose that stable inhibition of Nrf2 expression by RNA interference increases the sensitivity of immortalized human skin cells against electrophiles (e.g. aldehydes and ketones). Electrophilic compounds are frequently present as ingredients in a wide range of consumer products. For instance, sunscreens may contain electrophiles such as oxybenzone, octylmethoxy cinnamate, isoamyl cinnamate and others with up to 10 % as the maximum concentration allowed. To verify our concept, we will examine the cytotoxicity of selected sunscreen components including cinnamal as positive control in human cell lines deficient or naive for Nrf2 expression. To this end, we will apply the WST-cell viability assay (Roche). Using genetically modified cells deficient for endogenous defence mechanisms against electrophiles may help to identify and characterize compounds present in consumer products and thereby posing a risk to human health.
Section overview
Subjects
- Toxicology
