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Imprint
Replacement of fetal calf serum in the embryonic stem cell test (EST) – Standardization of culture conditions for proliferation and differentiation of embryonic stem cells of the mouse
Project
Project code: BfR-ZEBET-02-1322-159
Contract period: 01.08.2004
- 30.07.2007
Purpose of research: Applied research
According to the EST protocol, proliferation and differentiation of embryonic stem cells (ESC) are performed in a culture medium containing 15 % - 20 % fetal calf serum. Unfortunately the quality of the serum differs from batch to batch. Therefore, different batches of serum have to be pre-tested before they can be used in the EST. In order to standardize the test system our aim is to establish serum-reduced and/or serum-free culture conditions for proliferation and differentiation of ESC. Serum-free conditions would considerably increase the level of standardization of the EST an important prerequisite for applications in High Throughput Screening Systems (HTPS).
Section overview
Subjects
- Special animal species
- Biotechnology
Framework programme
Funding programme
Excutive institution
BfR - Centre for Documentation and Evaluation of Alternatives to Animal Experiments (BfR - ZEBET)
