Development of a test system to determine the sprouting resistance of triticale grown for bio-ethanol production, Subproject 2 Creation and propagation of a RIL population and carrying out the challenge test and determination of alpha-amylase activity

Project

Project code: 22023112
Contract period: 01.06.2013 - 31.08.2016
Budget: 128,765 Euro
Purpose of research: Applied research

Improving preharvest-sprouting resistance is of high economic importance, because this trait may be used to significantly reduce the risk of quality losses in years with adverse weather conditions shortly before harvest, Genetic improvement of triticale via plant breeding is the only promising strategy to this end. The aim of this project is to improve selection for preharvest sprouting in triticale while maintaining a high enzymatic activity in the ripening grain. For this an efficient and standardised test method for sprouting resistance has to be developed. Furthermore, a larger panel of triticale lines will have to be characterised in respect to sprouting resistance. At the phenotypic level, this will be accomplished both by assessing sprouting and estimating enzymatic activitiy via NIRS. At the genetic levele, molecular markers will be assessed in a QTL approach. The workplan comprises (i) developing a test method and its use in QTL analysis; (ii) evaluating the genetic variation for preharvest-sprouting resistance in a current panel of triticale cultivars; and (iii) devising an optimised selection strategy to improve sprouting resistance in the breeding process.

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