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A comprehensive proteogenomic analysis of Brucella to understand the epidemiology biology, virulence mechanisms, and host-pathogen interaction (BruceGenoProt)
Project
Project code: 2821ERA27D
Contract period: 01.04.2021
- 31.03.2024
Budget: 109,344 Euro
Purpose of research: Experimental development
Keywords: bacteriology, diagnostics, microorganisms, prevention, animal species comprehensive, animal health, zoonosis
Infectious animal diseases devastate the world’s communities, international trade, global food safety and public health. The animal health sector continues to suffer from a high prevalence of various contagious animal diseases, among which is brucellosis, one of the most frequently encountered bacterial zoonosis spread worldwide. Brucellae have been isolated from wildlife species, carrier hosts and non-classical hosts. However, livestock infection concerning wildlife contact and environmental contamination cannot be assessed. Moreover, several aspects of their biology, host/pathogen interaction and virulence mechanisms are not understood yet. To explain its epidemiology, virulence mechanisms, and host specificity, a better understanding of the genome, proteome and metabolome of brucellae will be needed. Thus, the project's main objectives are to assess the environment and wildlife's role in the transmission and dissemination of brucellosis and unravel several enigmatic aspects of brucellae utilizing various proteogenomics approaches. A comprehensive evaluation of the differences in B. abortus and B. melitensis genomes isolated from different hosts using Next Generation Sequencing (NGS) technology will provide an overview of the unique genes and virulence factors in each species. As a second task, we want to carry out a detailed characterization of the B. melitensis and B. abortus pan-proteome to understand the consequences of regulatory processes on microbes' protein composition. Experimental infection of cell lines originating from cows and sheep with B. abortus and B. melitensis will be carried out, and the outcomes will be assessed by RNA sequencing. We expect to develop a cgMLST scheme useful for epidemiological investigations and tracing back the sources of brucellosis in domestic ruminants.
Section overview
Subjects
- Animal health
Funding programme
Excutive institution
Friedrich-Loeffler-Institute - Federal Research Institute for Animal Health (FLI)
