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SPP 1090 AG 2: Highly Time-Resolved Study of Structural and Isotopic (d 13C) Variations within Lipid Fractions derived from a Naturally Marked Tschernosem
Project
Project code: DFG SPP 1090
Contract period: 01.01.2000
- 01.01.2006
Purpose of research: Basic research
Lipids are assumed to be of high importance for the stabilization of organic matter in soils. However, soil lipid investigations applying modern structural chemical and isotopic methods are still scarce. In this study a combination of these methods should give simultaneous information about the sources (plants, bacteria, fungi) and the conversion rates (d 13C) of the lipids on a molecular level. A change in land usage from a rye- (C3-) to a corn-monoculture (C4-plant) marked the newly added C4-biomass structurally and isotopically. The utilization of retained samples facilitates to study isotopic and compositional trends over the last four decades of this agricultural outdoors experiment in great detail. Soil lipids will be subjected to a combination of modern structural, spectroscopic, and isotopic analysis techniques of the soil chemistry, organic geochemistry and biochemistry. Analysis will be made using total soil samples and selected grain size fractions. Soil samples will be separated with the use of automated sequential liquid chromatography into the following fractions: a) aliphatics, b) aromatics, c) ketones/alcohols, d) fatty acids, e) basic lipids and f) high polar biopolymers. All fractions will be analysed for structural composition (13C NMR, GC-MS). Fractions a), c) and d) will be characterized for compound specific d 13C-variability (GC-irmMS). GC-irmMS investigations will be performed in collaboration with the MPI-Jena.
Section overview
Subjects
- Soil science
